Novel hptlc method for simultaneous determination of co-enzyme Q10 and a-tocopherol in bulk and pharmaceutical formulation
By: Kulkarni, Manasi B
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Contributor(s): Joshi, Anagha M | Patil, Rohini V.
Publisher: M. P. Innovare Academic Sciences Pvt Ltd 2018Edition: Vol. 10(10), July-August.Description: 134-141.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
International journal of pharmacy and pharmaceutical scienceSummary: Objective:HPTLC Method for Simultaneous quantification of co-enzyme Q10 and α-tocopherol in bulk and capsule dosage form was developed and validated as per International Conference on Harmonization [(ICH) Q2 (R1)] guideline. Methods:The chromatograms were developed using a mobile phase of Toluene: ethyl acetate: chloroform (10:1:2 v/v/v) on Pre-coated silica 60F254 plates and quantified by densitometric absorbance mode at 280 nm. Results:The Rf values were 0.77 and 0.87 for co-enzyme Q10 and α-tocopherol, respectively. The linearity of the method was found to be in the concentration range of 0.6μg-1.8 μg/band for α-tocopherol and 2 μg-6 μg/band for co-enzyme Q10. The limits of detection and quantification were 0.3154 and 0.9559 μg/band for α-tocopherol and 3.441 and 10.42 μg/band for co-enzyme Q10. Conclusion:Developed densitometric method was found to be robust, precise, accurate, and rapid and can be used to analyse fixed-dose capsule samples of co-enzyme Q10 and α-tocopherol.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
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Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2018432 |
Objective:HPTLC Method for Simultaneous quantification of co-enzyme Q10 and α-tocopherol in bulk and capsule dosage form was developed and validated as per International Conference on Harmonization [(ICH) Q2 (R1)] guideline. Methods:The chromatograms were developed using a mobile phase of Toluene: ethyl acetate: chloroform (10:1:2 v/v/v) on Pre-coated silica 60F254 plates and quantified by densitometric absorbance mode at 280 nm. Results:The Rf values were 0.77 and 0.87 for co-enzyme Q10 and α-tocopherol, respectively. The linearity of the method was found to be in the concentration range of 0.6μg-1.8 μg/band for α-tocopherol and 2 μg-6 μg/band for co-enzyme Q10. The limits of detection and quantification were 0.3154 and 0.9559 μg/band for α-tocopherol and 3.441 and 10.42 μg/band for co-enzyme Q10. Conclusion:Developed densitometric method was found to be robust, precise, accurate, and rapid and can be used to analyse fixed-dose capsule samples of co-enzyme Q10 and α-tocopherol.
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